Development of HPLC and UV spectrophotometric methods for the determination of ascorbic acid using hydroxypropyl-B-cyclodextrin and triethanolamine as photostabilizing agents

CLAUDIA GARNERO; MARCELA LONGHI

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Año: 2010 vol. 659 p. 159 - 159

font size="1" face="GulliverRM"> In this study, the effect of complex formation with triethanolamine (TEA) alone and in combination with hydroxypropyl-b-cyclodextrin (HP-b-CD) on the photostability of ascorbic acid was evaluated for exposure to artificial and diffuse daylight. The first-order rate constants for the photodegradation reactions were determined. The data obtained showed that these complexes strongly reduced the photodegradation process with an 11- and 35-fold increase in the photostability of ascorbic acid, depending of the ligand concentration and the irradiation source. The multicomponent complex gave a significantly better stabilization for exposure to light than TEA alone. Due to the fact that the complexation extended the exposure of ascorbic acid to light (without m