STUDY OF MECHANISMS UNDERLYING THERMOTOLERANCE ACTIVITY INDUCED BY CHLOROGENIC ACID IN C elegans

ANDREA CARRANZA; RAMÓN ASIS

Buenos aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de investigación Bioquímica y biología molecular y 9 sociedades mas

cell survival by providing fundamental building blocks tomaintain energy homeostasis during starvation or stressful situations. On the other hand, hypoxia-inducible-factor (HIF)-1, a mastertranscriptional regulator of adaptation to hypoxia, plays a centralrole in stress resistance. In these sense, in the present study wehave proposed to investigate the relationship between CGA, HIF1 and autophagy. Results showed that CGA was unable to conferresistance to thermal stress in worms with loss of function of HIF1 (ZG31) or with autophagy defects (cdc-48.2(tm659)). Moreover,preincubation of N2 worm with CGA before to be expose to thermalstress showed an increment in autophagy flux and HIF-1 activity. Finally, interactions between HIF-1 and autophagy underlying in CGAactivity were explored by RNAi assay. Here, we demonstrated thatautophagy and HIF-1 are mechanisms necessaries for CGA activity.All together, these preliminary results tend to explain the molecularmechanism that CGA activates in C elegans and evidence a possible hormetic response.