Determining Autoantibodies to Thyroid Peroxidase and Thyroglobulin in a Paediatric Population

SILVANO, L.; PAEZ NUÑEZ, A.; SOBRERO, G.; OCHETTI, M.; SCHVAB, G.; TARIFA, C.; TESTA, G.; BOYANOVSKY, A.; MARTIN, S.; MIRAS, M.; MUÑOZ, L.; MARINO, R.; PEREZ, N.; MIRAS, M.; MUÑOZ, L.

Cartagena de Indias

Encuentro; XXII Annual Meeting of the Sociedad Latino-Americana de Endocrinología Pediátrica (SLEP); 2011

Sociedad Latinoamericana de Endocrinología Pediátrica (SLEP)

Introduction and Objectives: Autoantibodies to thyroid Peroxidase and Thyroglobulin are markers of thyroid autoimmunity and can be quantified by different assays. It is necessary to establish reference values for each method in paediatric populations for an effective use in the study of autoimmune diseases (AD). Aims: To determine the cut-off levels of TPOAb and TgAb for two methods in healthy children. To establish the diagnostic concordance and theprevalence in different AD. Methods: We determined TPOAb and TgAb in 616 samples using ECLIA-Roche and CLIA-Siemens. We analyzed 229 healthy children and 387 patients with associated autoimmune diseases. Cut-off levels, diagnostic concordance and prevalence were determined. Results: Cut-off levels (IU/ml) are presented in table. TPOAb and TgAb were more prevalent in Hyperthyroidism,Turner Syndrome and Diabetes Mellitus. We observed significative differences in the diagnostic concordance of TgAb measurement (p = 0.0003). The highest TSH levels were observed with positive autoantibodies for each method. (p < 0.01). Conclusion: Our results allowed us to establish cut-off levels for the methods used in pediatric patients and confirmed the high prevalence of these antibodies in autoimmune diseases.