Paediatric reference intervals for FSH, LH, estradiol, testosterone, DHEA-S and cortisol by electrochemiluminescence

TARIFA, C.; SILVANO, L.; AGUIRRE, C.; SOBRERO, G.; LESCURAT, MP.; COLLET, I.; OCHETTI, M.; MARTIN, S.; MIRAS, M.; MUÑOZ, L.

Milán

Encuentro; 9th Joint Meeting of Paediatric Endocrinology; 2013

ESPE

ABSTRACT Background: The gonadotropin and steroid values obtained in various laboratories are often not comparable because of methodological differences. It is important to have normal data that are specific for the methods being used in the laboratory performing the test. Objective and hypotheses: To determine the reference values for FSH, LH, estradiol (E2), testosterone (To), DHEA-S and cortisol in our population pediatric. Methods: 693 healthy neonates and infants between 2 to 365 days were recruited. The blood samples were collected for establishing age and sex-stratified reference intervals for FSH, LH, E2, To, DHEA-S and cortisol using Electrochemiluminescence, Cobas e 601 analyzer. Results: The results are shown in table 1 2-59 days n:345 60-179 days n:248 180-365 days n:100 Female Male Female Male Female Male 2.5th - 97.5th 2.5th - 97.5th 2.5th - 97.5th 2.5th - 97.5th 2.5th - 97.5th 2.5th - 97.5th DHEA-S (ug/dL) 12.8 - 275.4 20.8 - 407.1 2.8 - 224.2 3.5 - 178.7 0.1 - 16.0 0.5 - 29.2 Cortisol (ug/dL) 0.5 - 23.0 0.6 - 19.6 0.2 - 23.4 1.0 - 26.0 1.2 - 23.9 0.9 - 23.4 To (ng/dL) 12.0 - 94.5 12.0 - 383.6 12.0 - 74.8 12.0 - 364.7 12.0 - 13.8 12.0 - 252.3 FSH (mIU/mL) 0.2 - 26.0 0.6 - 7.0 0.7 - 23.3 0.5 - 4.6 0.8 - 18.3 0.2 - 2.7 LH (mIU/mL) 0.1 - 3.5 0.4 - 10.1 0.1 - 3.1 0.2 - 7.6 0.1 - 0.9 0.1 - 3.0 E2 (pg/mL) 12.0 - 71.0 12.0 - 70.3 12.0 - 75.0 12.0 - 59.8 12.0 - 56.9 12.0 - 57.7 [Table 1: DHEA-S, Co, To, FSH, LH and E2 percentile] Conclusions: This reference values specific for age and sex may help in this period of life to increase the diagnostic power of this parameters for the as- sessment of endocrine disorders.