The ERalpha membrane pool modulates the proliferation of pituitary tumours

SOSA, LILIANA DEL V; PETITI, JUAN P; PICECH, FLORENCIA; CHUMPEN, SABRINA; NICOLA, JUAN P; PEREZ, PABLO; DE PAUL, ANA; VALDEZ-TAUBAS, JAVIER; GUTIERREZ, SILVINA; TORRES, ALICIA I

JOURNAL OF ENDOCRINOLOGY

BIOSCIENTIFICA LTD

Año: 2018

0022-0795

he molecular mechanisms underlying the ERα nuclear/cytoplasmic pool that modulates pituitary cell proliferation have been widely described, but it is still not clear how ERα is targeted to the plasma membrane. The aim of this study was to analyse ERα palmitoylation and the plasma membrane ERα (mERα) pool, and their participation in E2-triggered membrane-initiated signalling in normal and pituitary tumour cell growth. Cell cultures were prepared from anterior pituitaries of female Wistar rats and tumour GH3 cells, and treated with 10nM of estradiol (E2). The basal expression of ERα was higher in tumour GH3 than in normal pituitary cells. Full-length palmitoylated ERα was observed in normal and pituitary tumour cells, demonstrating that E2 stimulation increased both, ERα in plasma membrane and ERα and caveolin-1 interaction after short-term-treatment. In addition, the Dhhc7 and Dhhc21 palmitoylases were negatively regulated after sustained st