A Carboxy-Terminal Monoleucine-Based Motif Participates in the Basolateral Targeting of the Na+/I– Symporter (NIS)

MARTÍN, MARIANO; MODENUTTI, CARLOS PABLO; PEYRET, VICTORIA; GEYSELS, ROMINA CELESTE; DARROUZET, ELISABETH; POURCHER, THIERRY; MASINI-REPISO, ANA MARÍA; MARTÍ, MARCELO ADRIÁN; CARRASCO, NANCY; NICOLA, JUAN PABLO

ENDOCRINOLOGY

Oxford Academic

Año: 2019 vol. 160 p. 156 - 156

0013-7227

he Na+/I? symporter (NIS), a glycoprotein expressed at the basolateral plasma membrane of thyroid follicular cells, mediates I? accumulation for thyroid hormonogenesis, and radioiodide therapy for differentiated thyroid carcinoma. However, differentiated thyroid tumors often exhibit lower I− transport than normal thyroid tissue (or even undetectable I− transport). Paradoxically, the majority of differentiated thyroid cancers show intracellular NIS expression, suggesting abnormal targeting to the plasma membrane. Therefore, a thorough understanding of the mechanisms that regulate NIS plasma membrane transport would have multiple implications for radioiodide therapy. Herein, we show that the intracellularly facing carboxy-terminus of NIS is required for the transport of the protein to the plasma membrane. Moreover, the carboxy-terminus contains dominant basolateral information. Using internal deletions and site-directed