The transcription factor NF-kB mediates thyrotropin-stimulated expression of thyroid differentiation markers

GEYSELS, RC; PEYRET, V; MARTI, M; NAZAR, M; REALE, C; BERNAL BARQUERO, CE; MIRANDA, L; MARTI, MA; VITO, P; MASINI-REPISO AM; NICOLA, JP

THYROID

MARY ANN LIEBERT INC

Lugar: New York; Año: 2021 vol. 31 p. 299 - 299

1050-7256

p class="MsoNormal" style="text-align: justify; line-height: 200%;">Background:The Nuclear Factor kappa-light-chain-enhancer of activated B cells (NF-κB)transcription factors are key regulators of cell survival, proliferation andgene expression. Although activation of NF-κB signaling in thyroid follicularcells following thyrotropin (TSH) receptor (TSHR) engagement has been reported,the downstream signaling leading to NF-κB activation remains unexplored. Here, wesought to elucidate the mechanism that regulates NF-κB signaling activation inresponse to TSH stimulation. Methods:Fisher rat-derived thyroid cell lines and primary cultures of NF-κB essential modulator (NEMO)-deficientmice thyrocytes were used as models. Signaling pathways leading to theactivation of NF-κB were investigated using chemical inhibitors andphospho-specific ant