Macroporous Monolithic Supports for Affinity Chromatography

RUBEN DARIO ARRUA; C. ALVAREZ IGARZABAL

WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Año: 2011 vol. 34 p. 1974 - 1974

font size="1" face="AdvScalaLF-R"> In the early 1990s, three research groups simultaneously developed continuous macroporous rod-shaped polymeric systems to eliminate the problem of flow through the interparticle spaces generally presented by the chromatography columns that use particles as filler. The great advantage of those materials, forming a continuous phase rod, is to increase the mass transfer by convective transport, as the mobile phase is forced to go through all means of separation, in contrast to particulate media where the mobile phase flows through the interparticle spaces. Due to their special characteristics, the monolithic polymers are used as base-supports in different separation techniques, those chromatographic processes being the most important and, to a greater extent, those involving the separation of biomolecules as in the case of affinity chromatography. This mini-review reports the contributions of several g