Preparation of adorbents applicable to Pseudobiospecific ligand affinity chromatography using different spacers and ligands

CECILIA ALVAREZ IGARZABAL; MIRIAM STRUMIA; HÉCTOR BERTORELLO

REACTIVE & FUNCTIONAL POLYMERS

Elsevier

Año: 1997 vol. 34 p. 103 - 103

1381-5148

n this paper, a series of different adsorbents for pseudobiospecific ligand affinity chromatography is ,presented. They were obtained using ligands as histidine, Cu*+- or Zn*+-chelating iminodiacetic acid and Cibacron Bllue F3GA dye, attached to poly(hutadiene-hydroxyethyhnethacrylate) (PB-HEMA) matrix. The histidine and metal-ion-chelating iminodiacetic acid were attached on matrix through epichlorohydrin (ECH) or 1,4-butanediol diglycidyl ether (BDGE) as spacers and activators, while dye was directly bound onto the matrix.