Immobilized metal ion affinity dendronized supermacroporous cryogels for Immunoglobulin G adsorption

SERGIO D. GARCÍA SCHEJTMAN; SANTIAGO MARZINI IRRANCA; CECILIA INÉS ALVAREZ IGARZABAL ; MARISA MARTINELLI

Mar del Plata

Simposio; XVI Simposio Latinoamericano de Polímeros (SLAP 2018); 2018

Sociedad Argentino de Polímeros

Cryogels are highly elastic three-dimensional materials consisting of a polymer network obtained under cryogenic conditions (cryogelation). The use of monolithic cryogel columns offers several advantages over conventional stationary phase in bioseparation, related to fast flow, low back pressure and simple scale-up. Their structure with large (1 at 250 μm) and highly interconnected pores (supermacropore) also provide high performances for the purification of proteins. Immobilized metal ion affinity chromatography (IMAC) allows the separation and purification of proteins possessing exposed electron donor groups on their surface aminoacid residues, based on their affinities for metal ions (Co(II), Zn(II), Cu(II) and Ni(II)).Moreover, the dendritic structures are multi-branched molecules with high density of functional groups, which improve multivalent interactions. Therefore, the dendronized polymers are an alternative to obtain dendritic properties, then the aim of this work was synthetized novel supermacroporous cryogels for Immunoglobulin G (IgG) recognition, using a dendritic monomer (DM) and immobilized Cu(II).