Novel polymeric nanoparticles loaded with new second generation photosensitizers with probable phototoxic action

M.S. GUALDESI; J. VARA; C.I. ALVAREZ IGARZABAL; C.S. ORTIZ

Cordoba

Otro; RICIFA 2020+1; 2021

UNITEFA

There are promising data on the use of poly(acrylamide) nanoparticles (PAA-NPs) in the development of third generation photosensitizers for application in antimicrobial photodynamic therapy. These nanoparticle delivery systems are suitable for biological applications and have potential for drug delivery due to their biocompatibility, chemical flexibility, easy preparation and low cost. The objective of this work was to obtain new PAA-NPs for the vehiculization of the monobrominated derivatives of Azure B (AzBBr) and Neutral Red (NRBr). These two dyes were found to have promising properties as photosensitizers. However, the use of nanotechnology was required to overcome difficulties in solubility, aggregation and stability in order to optimize their photodynamic efficiency. The combination of different proportions of N-isopropylacrylamide allowed to obtain five new vehicle systems. These systems were loaded with AzBBr and NRBr and characterized according to their morphology, size, polydispersity index and zeta potential. The new third generation photosensitizers showed better photochemical reactivity and chemical stability than the free photosensitizers. The use of this vehiculization strategy improved the properties of the second generationphotosensitizers (AzBBr and NRBr) studied. This improvement in their properties makes them good candidates for potential phototherapeutic applications against numerous pathologies.